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Biocatalytics KRED 101 has been shown to selectively reduce the (6R,9S) enantiomer leaving behind the desired (6S,9R) enantiomer. This novel reaction is the first demonstration of a high yielding
R. 22.90. -3. B8. KRED 124. R. 29.10. -84.
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103. 104. 104. 104. 104. 105.
101, DK0002027895, 3,5NDASDROOA44, Nordea Kredit Realkreditaktieselskab 34, DK0030321088, Kred Alt II 2018, Nordea Bank AB (publ), 169,082,757
ordenligt, 5719, -. 102.
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2016. 0,244. 2002. 0,185. 0, 108.
Nej. Nej. Nej. Nej. Nej. K o rteb o. F. :102. M o. Biocatalytics KRED 101 has been shown to selectively reduce the (6R,9S) enantiomer leaving behind the desired (6S,9R) enantiomer. This novel reaction is the first demonstration of a high yielding (44% versus 50% maximum theoretical yield) highly stereoselective (>99% ee) resolution of a bicyclic ketone, via enzymatic reduction using a
The five natural enzymes (KREDs 20–24 in the table on the next page: KRED-101, KRED-119, KRED-130, KRED-NADH-101 and KRED-NADH-110) work poorly with IPA and therefore use glucose and glucose dehydrogenase (GDH) instead of IPA for cofactor recycling.
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Also, intermediate (XV) can be produced by enzymatic reduction of ketone (XI) using KRED-101 in the presence of GDH, NADP, KOH and PEG-400, KRED-X1.1-P1F01 in the presence of glucose and NAD in DMSO/i-PrOH or KRED-X1.1-P1B06, KRED-X1.1-P1F01 or KRED-X1.1-P1H10 in the presence of NADP in DMSO/i-PrOH or i-PrOH (11,12).
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